Search Repository UNIVERSITAS MH.THAMRIN

Introduction: Lipid peroxidation, one of known indices of oxidative stress, is documented in various diseases. secondary oxidation products such as malondialdehyde (MDA) is commonly measured to observe lipid peroxidation. In this study, a spectrophotometric method was evaluated to measure thiobarbituric acid reactive substances (TBARS) with high sensitivity. MDA is a known biomarker of oxidative status in a biological system. This study aimed to optimization standard of MDA using tetraethoxypropane (TEP) 97% (FW=220.3). Methods: The method is based upon reaction of malondialdehyde (MDA) and TBA in the glacial acetic acid medium. This research consists of two phases: first, making stock of TEP; and the second phase was testing the concentration of TEP for finding the standard curve of MDA before used in diagnostic of lipid peroxidation. Results: Result showed the concentration 1,875-60 uM of TEP can form a precise standard curve. Conclusion: In the concentration 1,875-60 uM of TEP can be used as a reference as standard of control in diagnostic of lipid peroxidation using TBARS method.