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Pneumonia is a form of acute respiratory infection that affects the lungs. Klebsiella pneumoniae is one of the most common causes. This study aimed to prevent adhesion of K. pneumoniae in the respiratory tract by using Lactobacillus bulgaricus in soyghurt. This was in vitro study done in the laboratory using HEp-2 cell lines with three processes of infection: pre-infection, co-infection and post-infection. This research aimed to get the best treatment between the concentration of L.bulgaricus in soyghurt against MIC value and growth inhibition zones of K. pneumoniae, and also to force anti-adhesives L.bulgaricus on the adhesion of K. pneumoniae strains to HEp-2 cell lines. This research consists of two phases: first, examine the MIC and effectiveness of L.bulgaricus in soyghurt against growth of K. pneumoniae strains. The second phase was testing the concentration of L. bulgaricus for inhibiting the adhesion of K. pneumoniae strains to HEp-2 cell lines according to the contact time of infection. Results showed the concentration 80% of L. bulgaricus in soyghurt can be bactericidal against K.pneumoniae strains, whereas the greatest inhibition zones was obtained by K. pneumoniae ATCC 700603 with concentration 90% amounting 19.167 mm. Treatment of L. bulgaricus in soyghurt with various concentrations 108 CFU/ml, 104 CFU/ml, and soy milk can inhibit the adhesion of K. pneumoniae strains to HEp-2 cell lines after 5 hours on the pre-infection and co- infection. The process of pre-infection, co-infection and post infection to K. pneumoniae ATCC 700603 in concentration of L. bulgaricus 108 CFU/ml after 5 hours decreased the adhesion of K. pneumoniae consecutive to 6.42%, 19.505% and 35.405 while K. pneumoniae S941 declined to 10.11%, 37.845% and 43.74%, and also K. pneumoniae CT1538 to 30%, 31.055% dan 55.875%. Inhibition of adhesion of K. pneumoniae on HEp-2 cell lines by L. bulgaricus in soyghurt depends on the strains of bacteria, the concentration, contact time of bacteria with epithelial cells, and the process of infection.